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In the realm of molecular biology and cell culture, maintaining a sterile environment is crucial for the successful growth and manipulation of cells. One of the most widely used antibiotic mixtures for preventing bacterial contamination in mammalian cell cultures is penicillin-streptomycin, often referred to as Pen-Strep. This combination not only protects cell cultures from bacterial infections but also plays a pivotal role in ensuring the overall health and viability of the cells throughout the experimental process.


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Amoxicillin works by inhibiting the synthesis of bacterial cell walls. Specifically, it binds to penicillin-binding proteins (PBPs) located inside the bacterial cell wall. By interfering with the cross-linking of peptidoglycan layers, amoxicillin compromises the integrity of the bacterial cell wall, leading to cell lysis and death. This mechanism is particularly effective against actively dividing bacteria, making it a preferred choice for treating infections caused by organisms that are sensitive to penicillin.


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